Manisha Mishra
All India Institute of Medical Sciences, India
Title: Association of MMP2 gene polymorphisms (-735C/T and -1306C/T) in preeclamptic patients in Indian population
Biography
Biography: Manisha Mishra
Abstract
Background & Aim: Imbalanced Matrix Metalloproteinase (MMP) expression, including MMP-2, has been demonstrated in pre-eclampsia. However, little is known about the effect of MMP-2 gene polymorphisms on hypertensive disorders of pregnancy. Therefore, we examined Matrix Metalloproteinase (MMP-2) gene polymorphisms (g.-735C/T) and its association with Preeclampsia (PE) and measured the levels of MMP-2 serum concentrations in PE. Method: 30 preeclamptic and 30 healthy pregnant women were enrolled from department of obstetrics and gynaecology, AIIMS, New Delhi after getting approval from Institute ethical committee. Genomic DNA was extracted from blood and amplified by PCR. MMP2 gene polymorphisms of -735C/T was detected by Restriction Fragment Length Polymorphism (RFLP). The levels of MMP2 in sera were measured by ELISA. Result: The maternal serum MMP2 levels was found to be more in PE patients than in control group (p=0.03). The increased frequency of CT genotype for MMP2 (-735C/T) Single Nucleotide Polymorphism was seen in PE patients as compared to control group. However the difference in genotype frequency was not statistically significant (p=0.35). Conclusion: These findings may help to understand the relevance of MMP-2 and its genetic polymorphisms to the pathophysiology of hypertensive disorders of pregnancy like preeclampsia and IUGR.